Automatic Analysis of CD68 (Macrophage) Glomerulus Occupancy Rate
Influence of CD68 (macrophage) on kidney tissue
CD68 (macrophage) permeation into the kidney glomerulus is a universal phenomenon seen in all progressive kidney diseases.
The glomerulus—a microscopic ball made of capillaries with small, open pores—governs the filtration function of the kidney. Approximately one million glomeruli exist in a single kidney. Macrophage permeation into the glomerulus leads to the phenomenon of fibrosis of kidney tissue.*
* What is fibrosis?
Fibrosis is the process in which connective tissue proliferates at an abnormal rate and replaces normal tissue. Specifically, in systemic sclerosis, an increase in the extracellular matrix of collagen fibers, and similar items within skin or internal organs, leads to their hardening. Fibrosis of the heart is a phenomenon in which an excess of extracellular collagen accumulates, leading to the hardening of the myocardium. This causes a decrease in the performance of the heart.
Related page: Quantification of Fibrosis
Analysis example of CD68 (macrophage) glomerulus occupancy rate
An example of the analysis of CD68 (macrophage) glomerulus occupancy rate is shown below. After HE staining was performed, a bird’s eye view of a kidney biopsy was captured. Normally, it is difficult to focus the image when the sample is thick, but Z-stack image capturing enables microscopy in which the entirety of the image is in focus.
Using the All-in-One Fluorescence Microscope BZ-X800
- Fully focused images even with thick samples.
- High-speed image stitching enables capturing of a bird’s eye view with high resolution.
- The thresholds can be set to fixed quantities automatically, which enables users to perform objective analysis.
- Here are some examples of using the All-in-One Fluorescence Microscope BZ-X800 in front-line research.
- [Myelodysplastic Syndromes (MDS)] Stitching, Sectioning and the Z-Stack Function as Decisive Arguments for the Acquisition of the BZ Fluorescence Microscope at the University Hospital of Düsseldorf
- [Neuropathology] The perfect solution for everyday patient diagnostics and clinical research at the Institute of Neuropathology in the Charité hospital in Berlin
- [Regenerative Medicine] BZ Series Provides Essential Imaging for Neural Stem Cell and Spinal Observation
- [Gene Therapy] Improving Research for the Development of Gene Therapy Drugs
- [Heart Disease Treatment] Developing Cell Sheets for Myocardial Regenerative Treatments
- [Cancer Treatment] Automated Fluorescence Microscope Transforms Process for Induced Cancer Stem Cell Research
- [Immune System] BZ Series Contributes to Understanding the Pathological Model of Asthma
- [Biomaterials] Promoting Efficiency in Research With Compact, User-friendly Microscopes